A fast and efficient procedure to produce scFvs specific for large macromolecular complexes.
نویسندگان
چکیده
We have expanded the application of antibody phage display to a new type of antigen: ribonucleoprotein (RNP) complexes. We describe a simple and efficient method for screening antibodies specific for large intact RNPs and individual components. We also describe a fast and easy method to overcome the abundance of amber stop codons in the positive phage clones. The resulting antibodies have been used in ELISA and Western blot analysis.
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ورودعنوان ژورنال:
- Journal of immunological methods
دوره 318 1-2 شماره
صفحات -
تاریخ انتشار 2007